News

New publication in project PEPTIMPRINT!

Development of Amino Acids Functionalized SBA-15 for the Improvement of Protein Adsorption

Molecules 202126(19), 6085; https://doi.org/10.3390/molecules26196085

Join an exciting project on photoactivable peptides for a targeted inhibition of SUMOylation in cancer cells

SUMOylation is a post-translational modification, which consists in the covalent conjugation of the small protein SUMO to lysine-side chains of proteins. SUMOylation has been involved in the regulation of most cellular processes. Because of its pleiotropic roles, SUMOylation is emerging as a promising target for the treatment of cancers. In particular, G. Bossis’s team (IGMM) has shown that SUMOylation plays a critical role in Acute Myeloid Leukemias (AML) response to therapies (chemotherapies, differentiation therapies and epigenetic therapies). However, the study of SUMOylation is hampered by the lack of tools to modulate its conjugation to target proteins. To fill this gap, we are, together with M. Amblard’s team (IBMM), developing a new kind of SUMOylation inhibitors.

As SUMOylation is highly dynamic and plays critical roles in numerous cellular processes, a general inhibition of SUMOylation can have pleiotropic effects and have deleterious consequences on normal cells. Finding ways to inhibit SUMOylation in a controlled manner would thus be highly valuable both to study the function of SUMOylation and for therapeutic intervention to limit potential side effects. On the basis of peptide-based SUMOylation inhibitors we already identified, we therefore propose to develop photoinducible inhibitors of SUMOylation.

The student recruited on this interdisciplinary project will be trained for peptide synthesis and incorporation of the photo-caged amino-acids at the IBMM and will test the activity of the photo-inducible peptides in cancer cells, in particular AMLs, at the IGMM.

Contacts: guillaume.bossis@igmm.cnrs.fr, muriel.amblard@umontpellier.fr

We are hiring ! Join an exciting project on Harnessing the ubiquitin/proteasome machinery to degrade oncoproteins using nanoscopic PROTACs

PROTACs are heterodimeric compounds displaying two ligands connected through a linker. One is a ligand for a protein of interest (POI) that is to be degraded, and the other one for an ubiquitin-E3 ligase (Ub-E3). PROTACs enable the hijacking of the ubiquitin-proteasome system to degrade a protein involved in a pathological process. To be fully functional, it must allow the recruitment of POIs and Ub-E3 in a finely controlled spatial arrangement. Therefore, the design and development of PROTACs can be tedious. Our project aims at solving this bottleneck by merging the PROTAC technology with the advantages of nanoscopic objects.

Contact : anthony.martin@umontpellier.fr

6 months internship – starting janv/feb 2021.

We are hiring ! Join an exciting project on SUMOylation Inhibitors as innovative treatment against Acute Myeloid Leukemia

SUMOylation, a post-translational modification of intracellular and particularly nuclear proteins, plays a critical role in the response of Acute Myeloid Leukemia (AML) to chemotherapies and differentiation therapies. AMLs are the only hematologic malignancies that have not benefited from a major therapeutic advance during the past 40 years. They are still treated via intensive treatments based on genotoxic agents, with very high fall rates and a poor 5-year survival rate of around 20%. To fill the urgent need for alternative therapies against AML, we are developing new classes of SUMOylation inhibitors using cutting-edge technologies. The Master 2 student that will be recruited on this multidisciplinary project will be in charge of the design and synthesis of peptide inhibitors at the Institut des Biomolécules Max Mousseron (IBMM) and will then evaluate their toxicity, ability to inhibit the SUMOylation and anti-leukemic efficiency at the “Institut de Génétique Moléculaire de Montpellier” (IGMM).

Contacts: baptiste.legrand@umontpellier.fr, olivier.coux@igmm.cnrs.fr

6 months internship – starting january-march

Ref:

Ubiquitin, SUMO, and Nedd8 as Therapeutic Targets in Cancer. Gâtel P, Piechaczyk M, Bossis G. Adv Exp Med Biol. 2020;1233:29-54. doi: 10.1007/978-3-030-38266-7_2.

A new review online! Peptides, polymers, lets bring them together!

After one year of hard work our extensive and exciting review on peptide containing polymers is online! The innovative bottom-up strategies recently developped are at the center of this publication. Have a nice read at https://www.sciencedirect.com/science/article/pii/S0079670021000241

New publication in project PEPTIMPRINT!

SOL-GEL PROCESS – THE INORGANIC APPROACH IN PROTEIN IMPRINTING

J. Mater. Chem. B, 2021 Jan. https://doi.org/10.1016/j.xphs.2020.10.019

IBMM peptide started a new project PEPTIMPRINT!

 The objective of Peptimprint is to set-up a ground-breaking technology to prepare highly specific tridimensional and functionalized imprints of biomolecules (peptides, proteins) by template-assembly, but in this case following the inorganic polymerization by sol-gel process. For that, a variety of original hybrid building blocks mimicking amino acids (diketopiperazine) will be synthetised and developed to be used as precursors (functional monomers) in the MIP synthesis.

Three prizes for the IBMM Peptide PhD students !

Margaux Clavié, Mathilde Guérin and Amandine Sandoval have respectively won the first price of popularizing scientific papers, my thesis in 400 words and best  oral communication during the PhD student congress of Perpignan. Congratulations !!

Back to back publications on self-assembing peptide polymers

Polymerization-Induced Self-Assembly (PISA) concept is based on the chain extension of homopolymers with a co-monomer to yield asymmetric block copolymers. During the chain extension with the second block, self-assembly is induced by the insolubility of the second block in the polymerization solution.

Self-assembling peptides (SAP) are a category of peptides, which undergo spontaneous assembling into ordered nanostructures.

In collaboration with ICGM and IEM institutes were the first to combine both strategies in order to obtain Self-Assembling Peptide—Polymer Nano-Objects via Polymerization-Induced Self-Assembly or SAP-PISA. In a first time SAP-PISA allowed us to obtain a range of original morphologies and macro architectures.

The way is open now for more structural exploration as well as for applications such as drug delivery.

Check more @

Self-Assembling Peptide—Polymer Nano-Objects via Polymerization-Induced Self-Assembly, Dao, L Vezenkov, G Subra, M.Amblard, M In, J-F Le Meins, F Aubrit, M-A Moradi, V Ladmiral, and M. Semsarilar* Macromolecules 2020, 53, 16, 7034–7043, https://doi.org/10.1021/acs.macromol.0c01260

Nano-assemblies with core-forming hydrophobic polypeptide via polymerization-induced self-assembly (PISA), T Dao, L Vezenkov, G Subra, V Ladmiral, M Semsarilar, Polym. Chem., 2020, Advance Article, DOI: 10.1039/D0PY00793E

New paper in European Polymer Journal !

Through a collaboration with the biopolymer team of IBMM , the peptide team describes for the first time the preparation of peptide-nylons hybrids. This novel work add another example of polymer-peptide to the list of studies lead by IBMM peptide team including peptide-silicone, peptide-NCA polymerization and Serine based switchable polymers obtained by ring opening polymerization of lactones.
Special congratulation to all the successive students, post-docs and colleagues who performed this long-lasting experimental work which started in 2016!